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The second ionization noticed while in the log kcat/Km profile could originate from other residues throughout the Lively site of NagA nonetheless it could also crop up within the protonation in the substrate itself Because the phosphate moiety has a p
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m vs. pH-profile for Zn-NagA is shown in Determine 2B. In this particular profile, catalytic exercise is dropped at superior and very low values of pH and the data had been in good shape to equation 4 in which two teams, in possibly the substrate or cost-free enzyme, with similar p